Research Paper Volume 9, Issue 5 pp 1359—1374

Aging-associated oxidative stress inhibits liver progenitor cell activation in mice

Figure 5. Neutrophils are required for inhibiting LPC proliferation in old mice. Old mice with CDE diet were i.p. injected with 500 μg of 2A3 (n/a) or 1A8 (anti-mLy6G) mAb every other day to deplete neutrophils. (A) Representative FACS scatterplots of liver-infiltrating neutrophils (CD11b+Gr-1high) in liver tissues from O-CDE mice treated with 2A3 or 1A8 mAb. Percentages of liver-infiltrating neutrophils were quantified. Results are mean ± SEM from three independent experiments (n = 3 mice per group). (B) Cumulative survival rates of mice were analyzed. n > 8 mice per group. (C) Representative FACS scatterplots and summarized percentages of EpCAM+CD45- cells in liver tissues from O-CDE mice treated with 2A3 or 1A8 mAb. Results are mean ± SEM from three independent experiments (n > 5 mice per group). *P < 0.05, **P < 0.01.