Research Paper Volume 9, Issue 6 pp 1552—1564

Melatonin prevents postovulatory oocyte aging and promotes subsequent embryonic development in the pig

Figure 6. Effects of melatonin on mitochondrial membrane potential in aged and melatonin treated oocytes by JC-1 detection assay. (A) Green-Fresh oocytes. (B) Green-Oocytes aged for 24 hr. (C) Green-IVA 24 hr oocytes treated with 2mM melatonin. (D) Green-Oocytes aged for 48 hr. (E) Green-IVA 48 hr oocytes treated with 2mM melatonin. (F) Red-Fresh oocytes. (G). Red-Oocytes aged for 24 hr. (H) Red-IVA 24 hr oocytes treated with 2 mM melatonin. (I) Red-Oocytes aged for 48 hr. (J) Red-IVA 48 hr oocytes treated with 2 mM melatonin. (K) Fluorescence pixel ratio (green/red) in fresh and aged oocytes. (L) The expression of mitochondria-related genes (Cytochrome C, SIRT1, Akt2 and Polg2) in fresh and aged oocytes. All graphs show mean ± s.e.m. Independent replicates were conducted with a minimum of 25 oocytes/replicate, at least 3 stable replicates were obtained. **P <0.01. Bar = 50 μm.