Figure 6. Effects of melatonin on mitochondrial membrane potential in aged and melatonin treated oocytes by JC-1 detection assay. (A) Green-Fresh oocytes. (B) Green-Oocytes aged for 24 hr. (C) Green-IVA 24 hr oocytes treated with 2mM melatonin. (D) Green-Oocytes aged for 48 hr. (E) Green-IVA 48 hr oocytes treated with 2mM melatonin. (F) Red-Fresh oocytes. (G). Red-Oocytes aged for 24 hr. (H) Red-IVA 24 hr oocytes treated with 2 mM melatonin. (I) Red-Oocytes aged for 48 hr. (J) Red-IVA 48 hr oocytes treated with 2 mM melatonin. (K) Fluorescence pixel ratio (green/red) in fresh and aged oocytes. (L) The expression of mitochondria-related genes (Cytochrome C, SIRT1, Akt2 and Polg2) in fresh and aged oocytes. All graphs show mean ± s.e.m. Independent replicates were conducted with a minimum of 25 oocytes/replicate, at least 3 stable replicates were obtained. **P <0.01. Bar = 50 μm.