Research Paper Volume 9, Issue 7 pp 1770—1824

The effects of graded levels of calorie restriction: XI. Evaluation of the main hypotheses underpinning the life extension effects of CR using the hepatic transcriptome

Figure 2. The mechanistic target of rapamycin (mTOR) signaling pathway obtained from the Ingenuity Pathway Analysis (IPA, www.qiagen.com/ingenuity) program. The normalized counts for each gene were correlated with the increase in calorie restriction (CR) level by Pearson correlation method. The pathway is colored based on a cut-off of an absolute correlation coefficient higher than 0.3. Red indicates a positive correlation with increasing CR level while green indicates a negative correlation. Circulating levels of insulin were significantly reduced in these CR mice [15] and this is indicated by a purple color.