Figure 1. Decreased enteroid forming efficiency and budding of crypts in enteroids from old compared to young animals. (A) Representative images of enterospheres and enteroids formed from crypts isolated from young and old mice and cultured in matrigel. Enterospheres are indicated by the black arrows. Buds are indicated by black triangles. Magnification: 10x, Scale bar: 100µm. (B) Quantification of enterospheres counted at day 1 that are able to grow into enteroids in matrigel culture. n=3 animals per group and 4-5 wells per animal, *p<0.05 Young vs Old, unpaired t test. (C) Quantification of enterosphere and enteroid complexity. n=3 animals per group and 4-5 wells per animal, *p<0.05 Young vs Old, unpaired t test.