Research Paper Volume 9, Issue 8 pp 1898—1915

Aging effects on intestinal homeostasis associated with expansion and dysfunction of intestinal epithelial stem cells

Figure 2. Increased jejunal villus height and number of Paneth cells per crypt with age. (A) Representative hematoxylin and eosin stained sections of jejunal cross-sections from young and old mice showing crypt and villus architecture. Images were taken at 10x magnification. Scale bar, 50µm. (B) Representative images of crypt sections stained for the Paneth cell marker Lysozyme and nuclear marker DAPI. Magnification: 40x, Scale bar: 20µm. n=6 animals per group. (C) Representative images of crypt sections stained for the Phloxine Tartrazine and the Goblet cell marker Alcian Blue. Magnification: 40x, Scale bar: 20µm. n=5 young and 6 old animals.