Research Paper Volume 9, Issue 8 pp 1898—1915

Aging effects on intestinal homeostasis associated with expansion and dysfunction of intestinal epithelial stem cells

Figure 6. Increased proportion of small intestinal epithelial cell apoptosis with age. (A) Representative image of crypt and villus sections stained for cleaved caspase-3. Magnification: 10x, Scale bar: 100µm . (B) Quantification of the number of cleaved caspase-3 positive cells per villus section (n=7 animals per group) and per crypt section (n=10 young and 9 old animals). *p<0.05 Young vs. Old, unpaired t test. (C) Representative image of crypt sections stained for cleaved caspase-3. Magnification: 40x, Scale bar: 20µm. (D) Quantification of the location of cleaved caspase-3 positive cells per 40 crypts per animal by position. Inlay shows method of identifying cell position within crypt. n=10 young and 9 old animals, *p<0.05 Young vs. Old, unpaired t test.