Research Paper Volume 9, Issue 10 pp 2052—2068

PKCζ-dependent upregulation of p27kip1 contributes to oxidative stress induced retinal pigment epithelial cell multinucleation

Figure 3. Involvement of Ca2+ signalling in RPE cell multinucleation. Intracellular calcium was blocked in ARPE-19 cells using BAPTA-AM. (A) calcium blocked cells. (B) calcium blocked + oxPOS treated cells. (C) oxPOS treated cells. Arrows indicate multinucleate RPE cells. (D) histogram showing the percentage of binucleate and multinucleate RPE cells following calcium chelation *, P < 0.05, **, P < 0.01, ***, P < 0.001 compared to oxPOS treated group. One-way ANOVA followed by Dunnett’s multiple comparison test. 50 cells were counted from three wells for each group. (E) PKCα inhibitor-treated RPE cells. (F) histogram showing the percentage of binucleate and multinucleate RPE cells after PKCα blockade **, P < 0.01 compared to oxPOS treated group. 50 cells were counted from three wells for each group.