Research Paper Volume 9, Issue 11 pp 2376—2396

An expression screen for aged-dependent microRNAs identifies miR-30a as a key regulator of aging features in human epidermis

Figure 6. MiR-30a overexpression decreases the functionality of reconstructed epidermis (REs). (A) Trans-epidermal water loss (TEWL) was measured for control or miR-30a overexpressing REs after doxycyclin treatment (B) Outside-in permeability was evaluated using the Lucifer Yellow assay for control or miR-30a overexpressing REs after doxycyclin treatment (C) Apoptosis TUNEL assay for control or miR-30a overexpressing REs after doxycyclin treatment. The apoptotic cells are labeled in green and indicated with white arrows. Scale bar = 25 μm. The Quantification of the % of apoptotic cells appears on the graph. Results are mean +/- SD from three independent samples. *P<0,05, **P<0,01, ***P<0,001.