Figure 1. NKILA is downregulated in ESCC tumor tissues and predicts poor prognosis of ESCC patients. (A) The expression levels of NKILA in ESCC tissues and corresponding noncancerous tissues was detected by qPCR. (B) Expression of NKILA in a panel of ESCC cancer cell lines and immortalized squamous epithelial cells. (C) The expression levels of NKILA in ESCC cancer tissues was normalized to that of corresponding noncancerous tissues. Data was presented as fold change of △Ct. The patients were assigned to high expression group and low expression group using the median fold change as cutoff value. Kaplan–Meier analysis of overall survival (D) and disease-free survival (E) in ESCC patients with low and high NKILA levels. Data in B represents the mean ± SD of three repeated experiments. *P < 0.05.