Research Paper Volume 10, Issue 1 pp 56—71

NKILA inhibits NF-κB signaling and suppresses tumor metastasis

Figure 4. NKILA suppresses ESCC cell migration in vitro and lung metastasis in vivo. (A) Silencing NKILA in Eca109 and Eca9706 cells resulted in elevated cell migration. Left panel was representative images and right panel was statistical quantification. (B) Expression level of E-cadherin, N-cadherin and Vimentin in Eca109 and Eca9706 cells after knockdown of NKILA was detected by qPCR. (C) Expression level of E-cadherin, N-cadherin and Vimentin in Eca109 and Eca9706 cells after knockdown of NKILA was detected by western blot. (D) Representative H&E staining of lung tissue sections. Scale bars: 50μm. (E) The micro-metastasis in the lung was numbered. Data in A and B represents the mean ± SD of three repeated experiments. Data in E represents the mean ± SD of six mice. *P < 0.05.