Research Paper Volume 10, Issue 1 pp 56—71

NKILA inhibits NF-κB signaling and suppresses tumor metastasis

Figure 6. NKILA inhibits activation of NF-κB signaling of ESCC cell. (A) Immunoblotting of p-IκBα and IκBα in Eca109 and Eca9706 cells after knockdown of NKILA. (B) Immunoblotting of p-IκBα and IκBα in KYSE30 and KYSE520 cells after overexpression of NKILA. (C) Immunoblotting of p65 in the cytoplasm and nucleus in Eca109 and Eca9706 cells after knockdown of NKILA. (D) Immunoblotting of p65 in the cytoplasm and nucleus in KYSE30 and KYSE520 cells after overexpression of NKILA. (E, F) Immunofluorescence of p65 in ESCC cells after manipulation of NKILA expression. The nucleus was counterstained with DAPI.