Figure 4. Ang II impairs α-processing of APP in senescent human BMECs. Cells were treated with Ang II with indicated concentrations for 24h. Cell lysates were collected for Western blot. Protein expression of (A) ADAM10, (D) APP and (E) BACE1 were measured, n=6-7. (C) Cell supernatants (1.5ml) were collected for sAPPα detection via a commercially available ELISA kit, n=11. *P<0.05, compared to control. (B) Senescent cells were treated with losartan (1μM) or PD123319 (1μM) for 1h, and then incubated with Ang II (0.5nM) for 24h. ADAM10 expression was measured, n=10. *P<0.05, compared to Ang II (0.5nM), #P<0.05, compared to Ang II (0.5nM) plus losartan (1μM). Data are presented as mean±SEM.