Research Paper Volume 10, Issue 1 pp 131—143

Rapamycin prevents the intervertebral disc degeneration via inhibiting differentiation and senescence of annulus fibrosus cells

Figure 3. Senescence of rabbit AFSCs cultured in growth medium with five different conditions for 6 days and stained by immunostaining. (A, F) Growth medium only (Control); (B, G) bleomycin 10 μg/ml (Bleo-10); (C, H) bleomycin 50 μg/ml (Bleo-50); (D, I) Bleomycin 50 μg/ml and rapamycin 25 nM (Bleo-50 + Rapa-25); (E, J) Bleomycin 50 μg/ml and rapamycin 50 nM (Bleo-50 + Rapa-50); (L) Semi-quantification of positive stained AF cells grown in five different conditions for 6 days. The images of F, G, H, I, J were enlarged areas of the boxes in images of A, B, C, D, E. The results indicated that bleomycin treatment induced the senescence of AF cells which was stained by red fluorescence. Rapamycin decreased cell senescence induced by bleomycin. White bars: 100 μm, Yellow bars: 50 μm. *p<0.05 compared to control, #P<0.05 compared to the cells treated with 50 μg/ml of bleomycin.