Research Paper Volume 10, Issue 3 pp 463—480

Protective effects of melatonin against nicotine-induced disorder of mouse early folliculogenesis

Figure 3. Melatonin preserves ovarian reserve in nicotine-exposed mice. (A) Representative IF for the germ cell marker Mvh in ovary tissue sections of control, intraperitoneally injected 1 mg /kg nicotine and intraperitoneally injected 1 mg /kg nicotine plus 1 μM melatonin mice after 7 dpp, 14 dpp and 21 dpp. (B) Quantification of the number of primordial, primary, secondary and antral follicles in control, intraperitoneally injected 1 mg /kg nicotine and intraperitoneally injected 1 mg /kg nicotine plus 1 μM melatonin mice ovaries after 7 dpp, 14 dpp and 21 dpp. All the experiments were repeated at least three times. * P < 0.05; ** P < 0.01.