Research Paper Volume 10, Issue 4 pp 645—657

AVE0991, a nonpeptide analogue of Ang-(1-7), attenuates aging-related neuroinflammation

Figure 6. AVE0991 elevates M2 activation makers in primary microglia. Primary microglia were directly isolated from the brain of 8-month-old SAMP8 mice. They were treated for 4 h with 100 ng/ml LPS with or without 4 h pre-incubation with AVE0991 (1×10-8, 1×10-7 or 1×10-6 M) in the presence or absence of A-779 (1×10-6 M) and were harvested and lysed for analysis. (A) The mRNA levels of Arg1 were investigated by qRT-PCR. (B) The mRNA levels of Il10 were investigated by qRT-PCR. (C) The mRNA levels of Retnla were investigated by qRT-PCR. Gapdh was used as an internal control, and data were expressed as a fold change relative to non-treated microglia. All data were analyzed by one-way ANOVA followed by Tukey’s post hoc test. Columns represent mean ± SD (n=4-6). *P<0.05 versus non-treated microglia. #P<0.05 versus microglia incubated with LPS alone. ⸹P<0.05 versus microglia treated with LPS+AVE0991 (1×10-7M).