Research Paper Volume 10, Issue 6 pp 1239—1256

Mitochondrial peptides modulate mitochondrial function during cellular senescence

Figure 6. Humanin and MOTS-c modulate the mitochondrial respiration. (A) Cellular oxygen consumption rate (OCR; pmole/min/total DNA) in non-senescence (quiescent) and senescent cells in the absence or presence of either HNG (a potent analogue of humanin with a glycine substitution, S14G) or MOTS-c. The basal respiration, spare respiratory capacity, and ATP production are calculated based on the sequential compound injection according to the manufacture’s instruction. (B) Representative western blots of carnitine palmitoyltransferase I (CPT1A) in non-senescence and senescent cells in the absence or presence of either HNG (a potent analogue of humanin with a glycine substitution, S14G) or MOTS-c. Quantification of carnitine palmitoyltransferase I (CPT1A) expression. Data are reported as mean ± SEM of three independent experiments. Significant differences were determined with one-way ANOVA followed by Tukey’s post hoc test. *p<0.05.