Figure 4. Effects of SAMR1 and SAMP8 mice fecal microbiota transplant on behavior in pseudo germ-free mice. (A) Schedule of fecal microbiota transplant on behavioral testing in pseudo germ-free mice. Mice were first treated by administering large doses of antibiotic solution for 14 consecutive days. Thereafter, mice were orally treated with fetal microbiota of SAMR1 and SAMP8 mice. MWMT was performed on days 29–33, and the probe trial was performed on day 34. On day 35, fecal samples were collected for 16S rRNA gene sequencing testing. (B) Body weight (Two-way ANOVA; Time: F2,14 = 25.59, P < 0.001; Group: F3,21 = 0.1132, P = 0.9514; Interaction: F6,42 = 0.6019, P = 0.7272.). (C) The trace graph of mice in MWMT. (D) Escape latency (two-way ANOVA; Time: F4,28 = 17.23, P < 0.001; Group: F3,21 = 3.27, P < 0.05; Interaction: F12,84 = 0.9568, P = 0.4961). (E) Escape path length (two-way ANOVA; Time: F4,28 = 32.04, P < 0.001; Group: F3,21 = 8.668, P < 0.001; Interaction: F12,84 = 0.7349, P = 0.7137.). (F) Platform crossing (one-way ANOVA; F3,28 = 8.745, P < 0.001.). (G) Time spent in each quadrant (one-way ANOVA; F3,28 = 9.133, P < 0.001). ANOVA: analysis of variance; MWMT: Morris water maze test. Data are shown as mean ± SEM (n = 8). *P < 0.05, **P < 0.01, ***P < 0.001.