Research Paper Volume 10, Issue 6 pp 1474—1488

microRNA-21 regulates astrocytic reaction post-acute phase of spinal cord injury through modulating TGF-β signaling

Figure 1. Astrocytes were activated and miR-21, TGF-β1, and CSPGs were upregulated after SCI. (A) Heat map of miRs with significantly altered expression in 3 days after SCI group compared with the sham group (n = 4). (B) Heat map of mRNAs with significantly altered expression in the 3 days after SCI group compared with sham group (n = 4). (C) qRT-PCR for miR-21 expression in sham and 3 days after SCI groups (n = 3). Data are expressed as mean ± SD. **P < 0.01 compared with sham group. Expression of TGF-β1 (scale bar: low magnification, 100μm; high magnification, 20μm) (D), GFAP (scale bar: low magnification, 100μm; high magnification, 20μm) (E) and CSPGs (scale bar: low magnification, 50μm; high magnification, 20μm) (F) in sham and 3 days after SCI group (n = 3). CSPGs, chondroitin sulfate proteoglycans; GFAP, glial fibrillary acidic protein; miR, microRNA; qRT-PCR, quantitative real-time polymerase chain reaction; SCI, spinal cord injury; SD, standard deviation; TGF-β1, transforming growth factor beta 1.