Research Paper Volume 10, Issue 8 pp 1884—1901

Anillin is required for tumor growth and regulated by miR-15a/miR-16-1 in HBV-related hepatocellular carcinoma

Figure 3. ANLN expression is associated with HCC cell viability. (A) Representative image of the immunofluorescence analysis of ANLN and Ki67 expressions in HCC tissue. (B) Correlation analysis of IHC staining scores for ANLN and Ki67 expressions in 53 HCC slices. (C) Western blotting analysis of the knockdown efficacy of ANLN shRNA in SMMC-7721 and QGY-7703 cells. (D) Q-PCR analysis of the efficacy of ANLN shRNA in SMMC-7721 and QGY-7703 cells. (E) Viability of SMMC-7721 and QGY-7703 cells after ANLN depletion was assessed by MTT assay at indicated times. (F) Representative image of NSI mice injected with control and ANLN knockdown SMMC-7721 cells. The red dashed line depicts the border of palpable xenograft tumors. (G) All tumors isolated from NSI mice are shown. (H-I) Growth curves (H) and weights (I) of xenograft tumors from NSI mice injected with control and ANLN knockdown SMMC-7721 cells. Changes in tumor volumes measured on the indicated days are shown. *P < 0.05; **P < 0.01; ***P < 0.001.