Figure 6. Sirt6 deletion impairs Notch signaling. 2.0 mm central corneal epithelial debridement wounds were induced in 8-week-old WT and Sirt6 KO mice and corneas were collected at indicated time points after injury. (A) Notch signaling downstream target Hes1 in the corneal was examined by qPCR in WT and Sirt6 KO corneas before injury (control), 24 hours (24h) and 2 weeks (2w) after injury. (B) Active Notch1 and Notch signaling downstream target Hes1 in the corneas were examined by Western blot in WT and Sirt6 KO corneas before or 24 hours after injury. *p<0.05 compared with WT, #p<0.05 compared with control (con), $p<0.05 compared with 24h. Two corneas were combined and served as one sample at each experimental condition (n=3-4, 6-8 corneas).