Research Paper Volume 10, Issue 8 pp 1964—1976

Circ-BPTF promotes bladder cancer progression and recurrence through the miR-31-5p/RAB27A axis

Figure 7. Circ-BPTF promotes BCa proliferation and migration through themiR-31-5p/RAB27A axis. (A) Schematic of predicted miR-31-5p binding sites in the 3’ UTR of RAB27A, with complementary pairs showed in black and mismatches showed in red. (B) Expression levels of RAB27A were detected following knockdown of circ-BPTF by qPCR. (C) Western blotting analysis of RAB27A in BCa cell lines upon knockdown of circ-BPTF and overexpression of miR-31-5p. GAPDH was used as a loading control. (D) miR31-5p decreases the luciferase activities of the wild-type RAB27A 3’ UTR reporter but not the luciferase activities of the mutant RAB27A 3’ UTR reporter. (E) Rescue experiment was performed to analyze RAB27A at protein level by western blotting. GAPDH was used as a loading control. **P<0.01.