Research Paper Volume 10, Issue 8 pp 2016—2036

Lycopene ameliorates oxidative stress in the aging chicken ovary via activation of Nrf2/HO-1 pathway

Figure 2. Protective effects of lycopene on D-gal-induced aged ovaries. (A) Effect of lycopene on D-gal-induced morphological changes of ovarian tissues, scale bar: 50 µm. (B) Effect of lycopene on D-gal-induced ultrastructural changes of granulosa cells: the four pictures in the lower row are the higher magnifications of the red squares from the four pictures in the upper row, respectively, scale bar: 2 µm (upper); 1 µm (lower), red arrowheads: fragmented mitochondria. (C) Effect of lycopene on D-gal-induced decline of BrdU index, scale bar: 20 µm; (D) Relative expression of proteins related to cell proliferation. (E) Effect of lycopene on D-gal-induced increase of TUNEL index, scale bar: 20 µm. (F) Relative expression of proteins related to pro-apoptosis (Bax) and anti-apoptosis (Bcl-xL). Values are expressed as the means±s.e.. Different lowercase letters indicate significant differences (P < 0.05).