Research Paper Volume 10, Issue 8 pp 2016—2036

Lycopene ameliorates oxidative stress in the aging chicken ovary via activation of Nrf2/HO-1 pathway

Figure 5. Protective effect of lycopene on the oxidative stress in the aging ovarian tissues was abolished by ML385. (A) Relative changes in the expression of Nrf2, pNrf2 and HO-1 after treatment with D-gal, ML385 alone or combined with lycopene. (B) Changes in the morphology of ovarian tissues after treatment with D-gal, ML385 alone or combined with lycopene, scale bar: 50 µm. (C and E) Changes in BrdU index in ovarian tissues after treatment with D-gal, ML385 alone or combined with lycopene, scale bar: 20 µm. (D) Relative changes in the expression of proteins related to cell proliferation and cell apoptosis in ovarian tissues after treatment with D-gal, ML385 alone or combined with lycopene. (E and G) Changes in TUNEL index in ovarian tissues after treatment with D-gal, ML385 alone or combined with lycopene, scale bar: 20 µm. (F) Changes in the levels of ROS in ovarian tissues after treatment with D-gal, ML385 alone or combined with lycopene. Values are expressed as the means±s.e.. Different lowercase letters indicate significant differences (P < 0.05).