Research Paper Volume 10, Issue 8 pp 2113—2121

MiR-146b inhibits autophagy in prostate cancer by targeting the PTEN/Akt/mTOR signaling pathway

Figure 2. MiR-146b influences the viability and proliferation of PCa cells. (A) Transfection with miR-146b mimics and inhibitor in PC3 cells markedly increased and decrease the expression of miR-146b compared with control cells. (B) MTT analysis results showed that upregulation of miR-146b promoted PC3 cells survival while downregulation of miR-146b could suppress cell viability of PC3 cells. 3-MA, inhibitor of autophagy, could rescue the cell viability following transfection of miR-146b inhibitor. (C) The results of flow cytometry showed that upregulation of miR-146b could promote PC3 cells proliferation while downregulation of miR-146b could suppress cells proliferation. 3-MA could also rescue the PC3 cell proliferation capacity following transfection of miR-146b inhibitor. (*P < 0.05, ***P < 0.001).