Research Paper Volume 10, Issue 8 pp 2170—2189

Exposure to Zinc oxide nanoparticles during pregnancy induces oocyte DNA damage and affects ovarian reserve of mouse offspring

Figure 3. nZnO induces DNA damage in fetal oocytes in vitro. (A) Representative double IF of pachytene and diplotene oocytes for SCP3 (red) and γH2AX (green) obtained from ovarian tissues cultured for 6 days. (B) Quantification of oocytes showing distinct γH2AX staining. (C) Representative WB for the indicated proteins from ovarian tissues cultured for 6 days; actin was used as loading control. (D) Representative double IF of pachytene and diplotene oocytes for SCP3 (red) and RAD51 (green). (E) Quantification of oocytes showing RAD51 foci.