Research Paper Volume 10, Issue 9 pp 2459—2479

MiR-665 aggravates heart failure via suppressing CD34-mediated coronary microvessel angiogenesis

Figure 5. Re-expression of CD34 reverses miR-665 induced endothelium dysfunction in vitro. (A) CD34 protein levels in treated HUVEC cells detected by Western blotting (n=4). (B) Migration evaluated by transwell experiment in HUVEC cells (n=3). (C)Tube formation determined on Matrigel in HUVEC cells (n=4). (D)Apoptosis measured by Annexin V/PI flow cytometric analysis in HUVEC cells (n=4). (E) Proliferation detected by EdU incorporation assays in HUVEC cells (n=4). Data are expressed as mean ± SEM.