Research Paper Volume 10, Issue 9 pp 2459—2479

MiR-665 aggravates heart failure via suppressing CD34-mediated coronary microvessel angiogenesis

Figure 7. Sp1 regulates miR-665 in vitro. (A) Sp1 protein level detected by Western blotting in TAC-induced heart failure mice. (B) Sp1 protein level detected by Western blotting in treated HUVEC cells. (C) Relative miR-665 expression in treated HUVEC cells measured by real-time PCR. (D and E) Dual luciferase activity assay in HEK293 cell cotransfected with pGL3 plasmids contain deferent length of 5′ flank of miR-665 and Sp1-siRNA. (F) A model to illustrate the role of miR-665 and CD34. Data are representative of three experiments, n=3. Data are expressed as mean ± SEM.