Figure 10. Schematic illustration of a nucleosome with DSB occupied by studied proteins of interest in G1 and late S phase of the cell cycle. (A) Schema of an intact nucleosome. (B) Nucleosome with wound DNA with DSB. (C) A pictorial illustration of histone signature and recruitment of the 53BP1 protein to DNA lesions in the G1 phase of the cell cycle. Immunoprecipitation result shows dimerization of 53BP1 protein. (D) An illustration of histone signature and recruitment of PCNA and BRCA1 proteins to DNA lesions in late S phase of the cell cycle. Panels represent a schematic illustration of the results.