Figure 2. AECs increase proliferation and NOS3 expression in AS. (A-B) AECs were purified by flow cytometry, based on CD31, shown by representative flow charts (A), and by quantification (B). (C-D) BrdU assay for sorted CD31+ AECs, shown by representative flow charts (C), and by quantification (D). (E) Representative western blots of CD31+ AECs. (F-H) Quantification of CCND1 (F), CDK4 (G) and NOS3 (H) protein levels in AECs. *p<0.05. N=10.