Research Paper Volume 10, Issue 10 pp 2695—2708

Role of alternative splicing of VEGF-A in the development of atherosclerosis

Figure 3. Macrophage infiltration and apoptosis increase in AS. (A-B) Aortic macrophages were purified by flow cytometry, based on F4/80, shown by representative flow charts (A), and by quantification (B). (C-D) Annexin V assay for sorted F4/80+ macrophages, shown by representative flow charts (C), and by quantification (D). (E) Representative western blots of F4/80+ macrophages. (F-G) Quantification of Bcl-2 (F) and cleaved caspase 3 (casps 3) (G) protein levels in F4/80+ macrophages. *p<0.05. N=10.