Research Paper Volume 10, Issue 10 pp 2911—2934

Nuclear Nox4 interaction with prelamin A is associated with nuclear redox control of stem cell aging

Figure 5. Nox4 post-translational modification in nuclei of AFSC. (A) Representative images showing DAPI (blue) and PML (red) of AFSC of group I and III. Tripled magnification image is present in white square of group I. (B) Representative images of AFSC group I (faster senescent cells) showing superimposing of DAPI (blue), farnesylated prelamin A (green) or Nox-4 (green) and PML (red). Tripled magnification image is present in white square of both. Scale bar= 10 µm. (C) Representative confocal image of AFSC group I samples labelled with Nox4 (green) and SUMO1 (red). Scale bar= 10 µM. (D) Western blot analysis of nuclear lysate (Nu), cytoplasmic lysate (Cy) and immunoprecipitation experiment of Nu and Cy with Nox4 antibody then revealed with anti-SUMO1. Data are representative of three independent experiments.