Research Paper Volume 10, Issue 10 pp 2911—2934

Nuclear Nox4 interaction with prelamin A is associated with nuclear redox control of stem cell aging

Figure 6. Effect of Anacardic Acid and MG132 treatment on Nox4 expression. (A) Representative image of Western blot analysis of AFSCs group II samples (intermediate senescent cells) used as control (0) or treated with anacardic acid for 24 h and then revealed with anti-Nox4. Tubulin detection was performed as a loading control. Data are representative of three independent experiments. On the right, representative images showing superimposing between DAPI (blue) and Nox-4 (green) of control or anacardic acid treated samples. Scale bar= 10 µm. (B) Representative image of Western blot analysis of AFSCs group II samples used as control (0) or treated with MG132 for 8 h and then revealed with anti-Nox4. Tubulin detection was performed as a loading control. Data are representative of three independent experiments. On the right, representative images showing superimposing among DAPI (blue), Nox-4 (green) or farnesylated Prelamin (green) and PML (red) of control or MG132 treated samples. Scale bar= 20 µm.