Research Paper Volume 10, Issue 10 pp 2954—2972

Melatonin prevents senescence of canine adipose-derived mesenchymal stem cells through activating NRF2 and inhibiting ER stress

Figure 2. ERS inhibitor 4-PBA attenuated the senescent phenotype in cADMSCs. (A) Relative levels of of ERS-related transcripts in 4-PBA or TM-treated cADMSCs. (B) Nucleus-to-cytoplasm ratio of 4-PBA or TM-treated cADMSCs. (C) SA-β-gal S of control, 4-PBA-treated and TM-treated cADMSCs. bar = 50 μm. (D) Doubling time of 4-PBA or TM-treated cADMSCs. (E) Telomerase activity of cADMSCs. (F) Relative telomere length of cADMSCs. (G) Relative levels of of SASP-related transcripts in 4-PBA or TM-treated cADMSCs. (H) Western blot quantification of ERS-related proteins (p-PERK and p-IRE1), SASP-related protein (IL6), and senescent markers (P16 and P21).