Figure 2. SASP induction in senescent RPESCs. Young (P3) and senescent (P16) RPESCs were maintained in culture for 48 h. The supernatant was analyzed for IL-6 (A), IL-12 (B), IL-17 (C), TNF-α (D), TGFβ1 (E), INF-γ (F), IL-4 (G), IL-10 (H), and IL-13 (I), by ELISA. Data are mean ± SD of 3 independent experiments. *P = from 0.021 to 0.041.