Research Paper Volume 10, Issue 12 pp 3662—3682

Long noncoding RNA B3GALT5-AS1 suppresses colon cancer liver metastasis via repressing microRNA-203

Figure 2. B3GALT5-AS1 suppressed colon cancer cell proliferation. (A) The expression of B3GALT5-AS1 in B3GALT5-AS1 stably overexpressed and control HCT116 cells was detected using qRT-PCR. (B) The expression of B3GALT5-AS1 in B3GALT5-AS1 stably depleted and control SW620 cells was detected using qRT-PCR. (C) Cell viability of B3GALT5-AS1 stably overexpressed and control HCT116 cells was detected using Glo cell viability assay. (D) Cell viability of B3GALT5-AS1 stably depleted and control SW620 cells was detected using Glo cell viability assay. (E) Cell proliferation of B3GALT5-AS1 stably overexpressed and control HCT116 cells was detected using EdU incorporation assay. The red color indicts EdU-positive cells. Scale bars = 100 μm. (F) Cell proliferation of B3GALT5-AS1 stably depleted and control SW620 cells was detected using EdU incorporation assay. The red color indicts EdU-positive cells. Scale bars = 100 μm. Results are displayed as mean ± s.d. of three independent experiments. **P < 0.01, ***P < 0.001, Student’s t-test.