Research Paper Volume 10, Issue 12 pp 3662—3682

Long noncoding RNA B3GALT5-AS1 suppresses colon cancer liver metastasis via repressing microRNA-203

Figure 3. B3GALT5-AS1 promoted migration, invasion, and EMT of colon cancer cells. (A) Cell migration of B3GALT5-AS1 stably overexpressed and control HCT116 cells was detected using transwell migration assay. Scale bars = 100 μm. (B) Cell migration of B3GALT5-AS1 stably depleted and control SW620 cells was detected using transwell migration assay. Scale bars = 100 μm. (C) Cell invasion of B3GALT5-AS1 stably overexpressed and control HCT116 cells was detected using transwell invasion assay. Scale bars = 100 μm. (D) Cell invasion of B3GALT5-AS1 stably depleted and control SW620 cells was detected using transwell invasion assay. Scale bars = 100 μm. (E) E-cadherin and N-cadherin mRNA levels in B3GALT5-AS1 stably overexpressed and control HCT116 cells were detected using qRT-PCR. (F) E-cadherin and N-cadherin protein levels in B3GALT5-AS1 stably overexpressed and control HCT116 cells were detected using western blot. (G) E-cadherin and N-cadherin mRNA levels in B3GALT5-AS1 stably depleted and control SW620 cells were detected using qRT-PCR. (H) E-cadherin and N-cadherin protein levels in B3GALT5-AS1 stably depleted and control SW620 cells were detected using western blot. Results are displayed as mean ± s.d. of three independent experiments. *P < 0.05, **P < 0.01, Student’s t-test.