Research Paper Volume 10, Issue 12 pp 3662—3682

Long noncoding RNA B3GALT5-AS1 suppresses colon cancer liver metastasis via repressing microRNA-203

Figure 6. B3GALT5-AS1 inhibited colon cancer liver metastasis. (A) B3GALT5-AS1 expression in different B3GALT5-AS1 stably overexpressed HCT116 cells clones was measured using qRT-PCR. Data are displayed as mean ± s.d. of three independent experiments. ***P < 0.001, Student’s t-test. (B) Indicated B3GALT5-AS1 stably overexpressed HCT116 cells were intra-splenic injected to establish liver metastasis. The amount of liver metastatic foci was assessed at the 42th day after intra-splenic injection using HE staining. Scale bars = 1000 μm. (C) The expression of B3GALT5-AS1 and miR-203 in liver metastatic foci formed by these indicated B3GALT5-AS1 stably overexpressed HCT116 cells was detected using qRT-PCR. (D) Immunohistochemical staining of Ki67 in liver metastatic foci formed by these indicated B3GALT5-AS1 stably overexpressed HCT116 cells. Scale bars = 50 μm. (E) The expression of ZEB2 and SNAI2 in liver metastatic foci formed by these indicated B3GALT5-AS1 stably overexpressed HCT116 cells was measured using qRT-PCR. (F) The expression of E-cadherin and N-cadherin in liver metastatic foci formed by these indicated B3GALT5-AS1 stably overexpressed HCT116 cells was detected using qRT-PCR. For B-F, data are displayed as mean ± s.d. of six mice in each group. **P < 0.01, NS, not significant, Mann-Whitney test.