Research Paper Volume 10, Issue 12 pp 3662—3682

Long noncoding RNA B3GALT5-AS1 suppresses colon cancer liver metastasis via repressing microRNA-203

Figure 7. Depletion of B3GALT5-AS1 promoted colon cancer liver metastasis in a miR-203-dependent manner. (A) miR-203 expression in B3GALT5-AS1 and miR-203 concurrently depleted and control SW620 cells was measured using qRT-PCR. Data are displayed as mean ± s.d. of three independent experiments. **P < 0.01, Student’s t-test. (B) B3GALT5-AS1 and miR-203 concurrently depleted and control SW620 cells were intra-splenic injected to establish liver metastasis. The amount of liver metastatic foci was detected at the 42th day after intra-splenic injection using HE staining. Scale bars = 1000 μm. (C) The expression of B3GALT5-AS1 and miR-203 in liver metastatic foci formed by B3GALT5-AS1 and miR-203 concurrently depleted and control SW620 cells was detected using qRT-PCR. (D) Immunohistochemical staining of Ki67 in liver metastatic foci formed by B3GALT5-AS1 and miR-203 concurrently depleted and control SW620 cells. Scale bars = 50 μm. (E) The expression of ZEB2 and SNAI2 in liver metastatic foci formed by B3GALT5-AS1 and miR-203 concurrently depleted and control SW620 cells was measured using qRT-PCR. (F) The expression of E-cadherin and N-cadherin in liver metastatic foci formed by B3GALT5-AS1 and miR-203 concurrently depleted and control SW620 cells was detected using qRT-PCR. For B-F, data are displayed as mean ± s.d. of six mice in each group. **P < 0.01, Mann-Whitney test.