Research Paper Volume 10, Issue 12 pp 3806—3820

Circular RNA EIF6 (Hsa_circ_0060060) sponges miR-144-3p to promote the cisplatin-resistance of human thyroid carcinoma cells by autophagy regulation

Figure 4. CircEIF6 regulated TGF-α through targeting miR-144-3p in the TPC1 and BHT101 cells with cisplatin treatment. (A) The target binding site between miR-144-3p and TGF-α was revealed and dual luciferase reporter gene assays was used to verify the target relationship between TGF-α and miR-144-3p (Right). *P < 0.05 compared with WT+miR control group. (B) TGF-α mRNA in the TPC1 and BHT101 cells with or without cisplatin treatment was measured after altering miR-144-3p expression and miR-144-3p had an inhibition role on TGF-α under cisplatin treatment. *P < 0.05 compared with control and #P < 0.05 compared with NC. (C) Transfection efficiency was identified after regulating circEIF6 expression in the TPC1 and BHT101 cells with or without cisplatin treatment. *P < 0.05 compared with control and #P < 0.05 compared with NC. (D) MiR-144-3p was decreased by circEIF6 overexpression and increased by circEIF6 knock-down. *P < 0.05 compared with control and #P < 0.05 compared with NC.