Research Paper Volume 10, Issue 12 pp 3834—3850

Tissue iron is negatively correlated with TERC or TERT mRNA expression: a heterochronic parabiosis study in mice

Figure 1. (A-F) Serum iron and other relevant indices in young and old mice. Serum iron (A), Tf saturation (B), UIBC (C), TIBC (D) and the contents of Tf, Ft-H, Ft-L, CP (E and F) were measured or calculated (Tf saturation and TIBC) in young (Y: 2-3 months) and old (O: 18-20 months) mice using commercial kits or western blot analysis as described in Methods and Materials. Data are presented as means ± SD (n=3). *p<0.05, **p<0.01 and ***p<0.001 vs. the control. (G-L) The contents of iron and the expression of iron metabolism proteins in the liver, kidney and heart of young and old mice. The contents of iron in the liver (G), kidney (H) and heart (I); the expression of TfR1, Fpn1, Ft-H, Ft-L and CP proteins in the liver (J and M), kidney (K and N) and heart (L and O) were determined in young (Y: 2-3 months) and old mice (O: 18-20 months) using western blot analysis or the methods described in Methods and Materials. Data are presented as means ± SEM (n=5). *p<0.05, **p<0.01 and ***p<0.001 vs. the control.