Figure 3. The effects of heterochronic parabiosis on the contents of iron and the expression of iron metabolism proteins in the liver of mice. The contents of iron (A), and the expression of TfR1, Fpn1, Ft-H, Ft-L and CP proteins (B – E) in the liver were determined in Y-Y, Y-O(Y), O-O and Y-O(O) mice using western blot analysis or the methods described previously. Data are presented as means ± SEM (n=3). *p<0.05 and **p<0.01 vs. the control.