Research Paper Volume 10, Issue 12 pp 3897—3909

Melatonin prevents deterioration in quality by preserving epigenetic modifications of porcine oocytes after prolonged culture

Figure 6. The effect of melatonin on NNAT expression and the glucose uptake of prolonged-culture oocytes. Porcine oocytes matured in vitro were continuously cultured in medium supplemented with or without 10−3 M melatonin for 24 h. (A) NNAT mRNA levels in the fresh, prolonged-culture, and prolonged-culture + Mel oocytes. (B) Representative images of fresh, prolonged-culture and prolonged-culture + Mel oocytes stained with NNAT antibody. (C) Quantification of the NNAT protein level. (D) Representative images of fresh, prolonged-culture and prolonged-culture + Mel oocytes stained with 2-NBDG. (E) Quantification of the 2-NBDG level. PC, prolonged-culture; Mel, melatonin; Scale bar=100 μm. The data are presented as the mean ± SEM of at least three independent experiments. *P<0.05, **** P<0.0001.