Research Paper Volume 10, Issue 12 pp 3910—3937

SIN-3 as a key determinant of lifespan and its sex dependent differential role on healthspan in Caenorhabditis elegans

Figure 1. sin-3 deletion causes reduction in lifespan. Kaplan-Meier survival curve showing the lifespan of sin-3;him-5 (dotted lines) and him-5(e1490) (solid lines) worms in (a) hermaphrodites and (b) males. Both hermaphrodites and males with sin-3 deletion manifest significant reduction in mean lifespan. (c) Movement rate in sin-3;him-5 hermaphrodites is compromised. Body bends are represented as strokes per minute. 20 hermaphrodites per strain were evaluated and the experiment was repeated thrice. (d) Movement rate in sin-3;him-5 males is compromised as compared to him-5 mutant males. Body bends are represented as strokes per minute. 20 males per strain were evaluated and the experiment was repeated thrice. (e) Pictures representing the tracks formed by nematodes of strains mentioned. (ns, non-significant; **p < 0.05; ****p < 0.001 and denotes the comparison with respect to him-5 (e1490); One-way ANOVA performed). The images were captured using Nikon stereoscopic zoom microscope at 5X zoom. Scale is equivalent to 200 pixels.