Research Paper Volume 10, Issue 12 pp 4152—4165

Mechanistic insight into hyaluronic acid and platelet-rich plasma-mediated anti-inflammatory and anti-apoptotic activities in osteoarthritic mice

Figure 3. Efficacy of HA+PRP treatment on apoptotic signaling and cell cycle modulatory proteins in chondrocytes. (A) Expression of p53, p21 and p-ERK were assessed by western blot after treatment in I+T conditioned medium in the presence of HA, PRP, and HA+PRP. (B) cyclin B, cyclin D, and cyclin E were assessed by western blot after treatment of I+T conditioned medium in the presence of HA, PRP, and HA+PRP. (C) Cell cycles distributions of chondrocytes. Percentages of sub-G1, G1, S, and G2 phases recorded by flow cytometry after treatment of I+T conditioned medium in the presence of HA, PRP, and HA+PRP. *p<0.05, **P<0.01, compared with the value in cells cultured in I+T using student t-test. The results are presented as mean ± S.D. for 15 independent experimental replicates.