Research Paper Volume 10, Issue 12 pp 4175—4187

Melatonin alleviates meiotic defects in fetal mouse oocytes induced by Di (2-ethylhexyl) phthalate in vitro

Figure 2. The effect of MLT on the formation of DSBs in fetal oocytes. (A)The immunofluorescence with Sycp3 (red) and γH2afx (green) in fetal oocytes. (B) The percentages of stronger, none and weaker γH2afx signal in oocyte in all MPI stages (control: 77.60 ± 5.04%, 22.40 ± 5.04%; DEHP: 87.24 ± 4.02%, 12.46 ± 4.02%; DEHP+MLT: 56.03 ± 9.52%, 43.97 ± 9.52%). (C) The percentages of stronger, weaker and none staining of γH2afx in pachytene and diplotene oocytes (control: 62.13 ± 3.37%, 35.92 ± 2.99%, 1.95 ± 0.78%; DEHP: 80.48 ± 1.53%, 17.41 ± 1.94%, 2.11 ± 0.78%; DEHP+MLT: 64.36 ± 2.39%, 35.64 ± 2.39%, 0.00 ± 0.00%). The results were presented as mean ± SEM. *P < 0.05, ** P < 0.01.