Research Paper Volume 10, Issue 12 pp 4175—4187

Melatonin alleviates meiotic defects in fetal mouse oocytes induced by Di (2-ethylhexyl) phthalate in vitro

Figure 3. Effects of MLT on HR in DEHP-exposed fetal oocytes. (A)The immunofluorescence with Sycp3 (red) and RAD51 (green) in fetal oocytes. (B) The percentages of more, less and none staining of RAD51 in all MPI stages (control: 32.48 ± 2.54%, 67.52 ± 2.54%; DEHP: 59.83 ± 7.44%, 40.17 ± 7.44%; DEHP+MLT: 33.13 ± 2.79%, 66.87 ± 2.79%). (C) The percentages of more, less and none staining of RAD51 in pachytene and diplotene oocytes (control: 36.27 ± 8.02%, 19.09 ± 1.03%, 44.64 ± 8.08%; DEHP: 44.24 ± 2.98%, 22.50 ± 4.28%, 33.26 ± 1.30%; DEHP+MLT: 32.97 ± 4.27%, 22.00 ± 1.03%, 45.03 ± 4.76%). The results were presented as mean ± SEM. *P < 0.05, ** P < 0.01.