Research Paper Volume 10, Issue 12 pp 4175—4187

Melatonin alleviates meiotic defects in fetal mouse oocytes induced by Di (2-ethylhexyl) phthalate in vitro

Figure 4. Effects of MLT on mismatch repair in DEHP-exposed fetal oocytes. (A)The immunofluorescence with Sycp3 (green) and MLH1 (red) in fetal oocytes. (B) The percentages of positive and negative MLH1 signal in oocytes (control: 23.63 ± 0.55%, 76.37 ± 0.55%; DEHP: 77.01 ± 4.41%, 22.99 ± 4.41%; DEHP+MLT: 55.04 ± 17.04%, 43.87 ± 17.08%). (C and D) The amounts of the MLH1 positive foci in pachytene (control: 7.91 ± 1.33; DEHP: 13.97 ± 0.95; DEHP+MLT: 6.74 ± 0.58) and diplotene (control: 12.11 ± 1.74; DEHP: 13.18 ± 1.16; DEHP+MLT: 7.58 ± 1.07) oocytes, respectively. The results were presented as mean ± SEM. *P < 0.05, ** P < 0.01.