Research Paper Volume 11, Issue 3 pp 950—973

Replicative senescent human cells possess altered circadian clocks with a prolonged period and delayed peak-time

Figure 4. Infection efficiency does not affect the oscillation patterns of luciferase. (A) BMAL1 mRNA levels in the proliferative (pro) and senescent (sen) TIG-3 cells were quantified by qPCR. Each sample was normalized by 18S rRNA. p = 0.47; n.s., not significant; by Student’s two-tailed t test; n=3. Three independent experiments were performed. (B) Relative oscillation patterns of luciferase in serially diluted virus-infected TIG-3 cells are shown. Highest intensity of each sample was set to 1. Two independent experiments were performed.