Figure 4. C2C12 cells overexpressing miR-34a secrete EVs with elevated levels of miR34a. (A) Confocal images of C2C12 cells transfected with a lentivirus overexpressing miR-34a. The virus contains a GFP reporter under a constitutive CMV promoter. Images show GFP expression in transfected cells. Blue staining represents nuclear DAPI staining. Scale bar = 20 µm. (B) Analysis of miR-34a expression in EVs from transfected and non-transfected cells shows a three-fold increase in miR-34a in EVs isolated from conditioned medium of transfected cells.