Research Paper Volume 11, Issue 6 pp 1791—1803

Muscle-derived miR-34a increases with age in circulating extracellular vesicles and induces senescence of bone marrow stem cells

Figure 7. Western blot for exosome markers and experimental design for in vivo treatment. (A) SGCA+ EVs isolated from serum of young (Y) and old (O) mice as well as from conditioned medium of normal C2C12 cells and cells overexpressing miR-34a are positive for the exosome markers CD63 and TSG101. (B) Timeline, EV doses, and outcome measures for in vitro BMSC treatments.